Dibawah ini adalah informasi produk dari RT PCR DTLite DNA Technology. Untuk diskusi produk bisa menghubungi kontak PT. Andaru Persada Mandiri via whatsapp 087777277740 atau telepon 0251-7504679.
Overview Product RT PCR DTLite DNA Technology
DTlite real-time PCR instrument is used for qualitative and quantitative analysis of DNA and RNA targets. It is an optimum choice for diagnostic laboratories with small or medium throughput workflow. The device can be used for qualitative and quantitative analysis in the fields of:
- Human genetics
- Gene Expression profiling
- Pathogen detection, quantification and genotyping
- Microbiome composition analysis
The software with user-friendly interface, common to all DT real-time PCR instruments, controls the device. Original software lowers the probability of mistakes and is used for sophisticated assays requiring multiple settings application.
Advantages of RT PCR DTLite DNA Technology
- Tubes Height Measurement system provides “openness” of the instrument to different types of plastic (low and high profile; flat and domed caps; strips, plates).
- Simultaneous detection of a fluorescent signal in all wells of the matrix provides uniformity of detection and high speed of the instrument.
- Narrow range filters (cross-talk cut-off function).
- No “edge effect” – the thermo-block moving and positioning system ensures its movement in the horizontal and vertical positions, which guarantees complete isolation of the reaction tubes from the influence of environmental factors such as light penetration into the optical path and temperature non-uniformity between the edge rows of the matrix and its central part.
- The hot lid module provides reliable heat contact between tubes and the thermal block plate maintaining temperature of the tube caps at the level of 105±1°С in order to prevent condensate formation on the tube caps, since it may cause substantial distortions in the luminous flux measurements.
- Data protection system provides stable and safe operation of the instrument in the event of an unstable power supply. All parameters of the current run are recorded in the instrument’s own memory.
- The software has a plenty of settings allowing advanced users to obtain specific data and finely tune instrument for a given task;
- Automated filling template in case of usage DNA-Technology PCR kits.
- Can be integrated into laboratory information systems (LIS) to store data in standard graphic and text formats.
- Ability to control multiple instruments from a single control computer increases lab productivity and allows you to simultaneously conduct a wide range of PCR tests using kits with different amplification programs.
- The narrow body and compact design allow you to place several instruments in a small area.
Detail Specifications of RT PCR DTLite DNA Technology
| General Characteristics | DTlite | |
| DTlite 4S1 | DTlite 5S1 | |
| Hot lid 105°С±1°С | ✔ | ✔ |
| Adjustable height of the tubes | ✔ | ✔ |
| Autonomous operation mode (without using a control computer) | ✔ | ✔ |
| Protection against mains voltage failures (automatic restart of the amplification program) | ✔ | ✔ |
| Pre-calibration of the device | ✔ | ✔ |
| No need for additional calibrations | ✔ | ✔ |
| Warranty period (24 months) | ✔ | ✔ |
| Post-warranty service | ✔ | ✔ |
| Line voltage 100-240 W | ✔ | ✔ |
| Power consumption max 550 W | ✔ | ✔ |
| Frequency 50/60 Hz | ✔ | ✔ |
| Size 210 × 480 × 310 mm | ✔ | ✔ |
| Mass 17 kg | ✔ | ✔ |
| Thermal unit characteristics | DTlite | |
| DTlite 4S1 | DTlite 5S1 | |
| Thermal block plate format | ✔ | ✔ |
| Aluminum matrix | ✔ | ✔ |
| Heating and cooling method with 6 Peltier thermoelectric elements | ✔ | ✔ |
| Forced cooling and heating of matrix | ✔ | ✔ |
| No «edge effect» | ✔ | ✔ |
| Number of wells in thermal block | 48 | 48 |
| Volume of reaction mixture (allowable), mcl | 10 – 100 | 10 – 100 |
| Volume of reaction mixture (recommended), mcl | Oct-50 | Oct-50 |
| Consumables type (separate tubes, stripes, microplates) | 0,2 ml | 0,2 ml |
| Isolated pseudo blocks | monoblock | monoblock |
| Independent temperature control of pseudo blocks with time-synchronous profile | - | - |
| Temperature gradient between pseudo blocks with independent control, °С | - | - |
| Thermal block temperature range 0-100°С | ✔ | ✔ |
| Temperature accuracy ±0,2°С | ✔ | ✔ |
| Temperature uniformity ±0,15°С | ✔ | ✔ |
| Maximum heating rate of thermal unit, °С/sec | 3,5 | 3,5 |
| Maximum cooling rate of thermal unit, °С/sec | 2,5 | 2,5 |
| Average heating rate, °С/sec | 3,3 | 3,3 |
| Average cooling rate, °С/sec | 2,1 | 2,1 |
| Temperature increment (discreteness 0,1 sec) | ✔ | ✔ |
| Time increment (discreteness 0,1 sec) | ✔ | ✔ |
| General characteristics of optical system | DTlite | |
| DTlite 4S1 | DTlite 5S1 | |
| Excitation source – LED | ✔ | ✔ |
| Number of excitation sources | 4 | 5 |
| Spectral range, nm | 470 | 470 |
| 530 | 530 | |
| 580 | 580 | |
| 630 | 630 | |
| 687 | ||
| CCD camera | ✔ | ✔ |
| CCD matrix | ✔ | ✔ |
| Number of fluorescence measuring channels | 4 | 5 |
| Detection for each channel, nm | 515 | 515 |
| 560 | 560 | |
| 620 | 620 | |
| 660 | 660 | |
| 731 | ||
| Simultaneous signal detection in each well | ✔ | ✔ |
| Light filters (absolute isolation of the optical channel from external light) | ✔ | ✔ |
| Eliminated cross-channel effect | ✔ | ✔ |
| Scanning time for all channels is no more than 20 sec. | ✔ | ✔ |
| “Threshold” level of fluorescence 0,05х10Е-12М | ✔ | ✔ |
| Calibrated dyes (per channel) | Fam | Fam |
| Hex | Hex | |
| Rox | Rox | |
| Су5 | Су5 | |
| Су5,5 | ||
| Compatibility with other fluorophores: | ✔ | ✔ |
| Fam – SybrGreen | ||
| Hex – VIC, R6G | ||
| No calibrators / need to calibrate | ✔ | ✔ |
| Dynamic range 9 lg | ✔ | ✔ |
| Melting curves analysis | ✔ | ✔ |
| Absolute and relative quantitative analysis | ✔ | ✔ |
| Software | DTlite | |
| DTlite 4S1 | DTlite 5S1 | |
| USB-devices connection | ✔ | ✔ |
| Computer interface (USB 2.0 high speed) | ✔ | ✔ |
| Integration to LIS | ✔ | ✔ |
| Results export as .xml, .txt | ✔ | ✔ |
| Operating system: Windows® 7, Windows® 8, Windows® 10, Windows® XP, Windows®Vista | ✔ | ✔ |
Sumber informasi produk didapat dari website principle langsung pada tautan berikut : dnatechnology
Demikian informasi produk RT PCR DTLite DNA Technology. Bagi anda yang membutuhkan diskusi cepat produk ini bisa menghubungi contact PT. Andaru Persada Mandiri via whatsapp 087777277740 atau telepon 0251-7504679. Link alamat kami sertakan pada Google Maps.
