Rekomendasi Alat PCR Untuk Klinik dan Rumah Sakit

Overview Product RT PCR DT-Prime

DTlite real-time PCR instrument is used for qualitative and quantitative analysis of DNA and RNA targets. It is an optimum choice for diagnostic laboratories with small or medium throughput workflow. The device can be used for qualitative and quantitative analysis in the fields of:

1. Human genetics;
2. Gene Expression profiling;
3. Pathogen detection, quantification and genotyping;
4. Microbiome composition analysis.

The software with user-friendly interface, common to all DT real-time PCR instruments, controls the device. Original software lowers the probability of mistakes and is used for sophisticated assays requiring multiple settings application.

Advantages of PCR DT-Prime

1. Tubes Height Measurement system provides “openness” of the instrument to different types of plastic (low and high profile; flat and domed caps; strips, plates).

2. The narrow body and compact design allow you to place several instruments in a small area.

3. Ability to control multiple instruments from a single control computer increases lab productivity and allows you to simultaneously conduct a wide range of PCR tests using kits with different amplification programs;

4. Can be integrated into laboratory information systems (LIS) to store data in standard graphic and text formats;

5. Automated filling template in case of usage DNA-Technology PCR kits;

6. The software has a plenty of settings allowing advanced users to obtain specific data and finely tune instrument for a given task;

7. Data protection system provides stable and safe operation of the instrument in the event of an unstable power supply. All parameters of the current run are recorded in the instrument’s own memory;

8. The hot lid module provides reliable heat contact between tubes and the thermal block plate maintaining temperature of the tube caps at the level of 105±1°С in order to prevent condensate formation on the tube caps, since it may cause substantial distortions in the luminous flux measurements;

9. No “edge effect” – the thermo-block moving and positioning system ensures its movement in the horizontal and vertical positions, which guarantees complete isolation of the reaction tubes from the influence of environmental factors such as light penetration into the optical path and temperature non-uniformity between the edge rows of the matrix and its central part.

10. Narrow range filters (cross-talk cut-off function);

11. Simultaneous detection of a fluorescent signal in all wells of the matrix provides uniformity of detection and high speed of the instrument;

Spesifikasi Alat PCR DT Prime Kapasitas 96 Sample

Thermal unit plate format:

• Monoblock (M1 version) – 96 0.2 ml tubes (12×8 arrangement)
• 3-section thermal unit (M3 version) – 96 0.2 ml tubes (12×8 arrangement)
• 6-section thermal unit (M6 version) – 96 0.2 ml tubes (12×8 arrangement)
• Monoblock (X1 version) – 384-well 0.045 ml plate (24×16 arrangement)

Consumables type For M version:

• PCR 0.2ml tubes (separate or in strips, 8 pieces each)
• 96-well 0.2 ml PCR plate

Consumables type For Х1 version:

• 384-well 0.045 ml PCR plate

Number of fluorescence measuring channels

• 4 (versions 4М1, 4М3, 4М6)
• 5 (versions 5М1, 5М3, 5М6 and 5X1)

Spectral range (nm) Excitation and detection for each channel

• Fam – 470/40 – 515/30 (compatible with SybrGreen)
• Hex – 530/20 – 560/20 (compatible with VIC, R6G)
• Rox – 580/30 – 620/30 (compatible with TAMRA)
• Cy5 – 630/20 – 660/20
• Cy5.5 – 687/20 – 731/30

Thermal unit temperature range (°C) : from 0 to 100
Temperature step (°C) : 0.1
Temperature accuracy (°C) : +/– 0.2
Maximum heating rate of thermal unit (°C/sec) : For M versions: 3.5 | For X versions: 2.5
Maximum cooling rate of thermal unit (°С/sec) : For M versions: 2.5 | For X versions: 1.5
Heating and cooling method : 6 Peltier thermoelectric elements
Excitation source : LED
Detector : CCD
Color display : 29×97 mm with 690×480 pix resolution and increased service life (at least 30,000 hours)
Hot lid temperature (°C) : 105
Line voltage (V) : 220
Power consumption (W) : Max 500
Frequency (Hz) : 50
Size (WxDxH) (mm) : 210x540x540
Mass (kg) : 27