A new instrument for real-time amplification and melting analysis suitable both for research studies as well as for diagnostics applications. Two thermoeletric Peltier elements ensure high accuracy of temperature regulation and noiselessness.
The unique design and project of the thermal block allows loading and unloading of test samples in a completely automated software-controlled way.
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Overview & Features
Specification
Overview & Features
Features Polymerase Chain Reaction
- 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well*
- Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips
- Possibility to use tubes with different heights with regulation of the matrix block
- Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera
- Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance or constant
- Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings
- Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis
- Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.
Optical Scheme Polymerase Chain Reaction
Specification
| Thermal block format | 96 test tubes of 0.2 ml (12 x 8) |
| Test tube type | 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a holder 12 x 8) |
| Range of thermal block temperature control | 0ºC…100ºC |
| Resolution of temperature setting | 0.1ºC |
| Absolute accuracy of temperature maintenance | ±0.2ºC |
| Uniformity of thermal block temperature | ±0.15ºC |
| Average heating rate of the thermal block within temperature range of 4…99ºC | 3.3ºC / s |
| Maximum heating rate of the thermal block within temperature range of 4…99ºC | 3.5ºC / s |
| Average cooling rate of the thermal block within temperature range of 99…55ºC | 2.1ºC / s |
| Maximum cooling rate of the thermal block within temperature range of 99…55ºC | 2.5ºC / s |
| “Hot cover” temperature | 105ºC ±1ºC |
| Actuating device of the thermal block | Peltier elements |
| Excitation source | Light-emitting diode (LED) |
| Detector | CCD (Charge Coupled Device) -matrix |
| Number of the fluorescence measurement channels | 4 or 5* |
| Excitation /detection wave length | 470/525, 532/570, 585/633, 633/670, 690/750 ** |
| Threshold sensitivity of each of the channels for solutions of standard fluorophores | 0.05x10E-12M |
| Computer interface | USB 2.0 High-speed |
| Power consumption | Not over 550 W |
| Overall dimensions, WxDxH | 210x540x540 mm |
| Preparation time after switching-on | Not over 5 minutes |
| Weight | 27kg |
| * The 5th channel is optional and available only after customer’s specific request | |
| ** The excitation/detection 690/750 wavelengths are for the 5th optional channel | |
